RPB2 Antibody (HL1043) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13698
Recombinant Monoclonal Antibody
![Western Blot: RPB2 Antibody (HL1043) [NBP3-13698]](https://resources.rndsystems.com/images/products/RPB2-Antibody-HL1043-Western-Blot-NBP3-13698-img0001.jpg "Western Blot: RPB2 Antibody (HL1043) [NBP3-13698]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1043 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for RPB2 Antibody (HL1043) - Azide and BSA Free
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698]
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] -
Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.
RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] -
Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] -
Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.
RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] -
Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] -
Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.
RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -
Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for RPB2 Antibody (HL1043) - Azide and BSA Free
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RPB2
Alternate Names
DNA-directed RNA polymerase II subunit RPB2, EC 2.7.7, EC 2.7.7.6, hRPB140, hsRPB2, POL2RB, polymerase (RNA) II (DNA directed) polypeptide B (140kD), polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA polymerase II subunit2, RNA polymerase II second largest subunit
Gene Symbol
POLR2B
Additional RPB2 Products
Product Documents for RPB2 Antibody (HL1043) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RPB2 Antibody (HL1043) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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