RRAS Antibody (OTI2B7)

Novus Biologicals | Catalog # NBP3-44764

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2B7
View all RRAS Primary Antibodies »

Product Specifications

Immunogen

Full length human recombiant protein of human RRAS (NP_006261) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for RRAS Antibody (OTI2B7)

RRAS Antibody (OTI2B7) Western Blot;RRAS Antibody (OTI2B7)[NBP3-44764]-

Western Blot;RRAS Antibody (OTI2B7)[NBP3-44764]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY RRAS ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRAS (1:2000). Positive lysates
RRAS Antibody (OTI2B7) Western Blot;RRAS Antibody (OTI2B7)[NBP3-44764]-

Western Blot;RRAS Antibody (OTI2B7)[NBP3-44764]-

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, # and RRAS-Knockout HeLa cells (KO,) were separated by SDS-PAGE and immunoblotted with anti-RRAS monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Applications for RRAS Antibody (OTI2B7)

Application
Recommended Usage

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RRAS

R-Ras is a member of the Ras superfamily of GTPases. Ras proteins function as signaling focal points that are activated by convergent signaling pathways intiated by extracellular stimuli (reviewed in Mitin et al, 2005). Activated Ras proteins in turn regulate a diversity of downstream cytoplasmic signaling cascades. Many members of the Ras superfamily are implicated in the regulation of hematopoietic cells, with roles in growth, survival, differentiation, cytokine production, chemotaxis, vesicle-trafficking, and phagocytosis. The Ras superfamily of proteins includes over 150 small GTPases (distinguished from the large, heterotrimeric GTPases, the G-proteins). It comprises six subfamilies, the Ras, Rho, Ran, Rab, Arf, and Kir/Rem/Rad subfamilies. They exhibit considerable overall amino acid identities, especially in the regions interacting with the guanine nucleotide exchange factors that catalyze their activation. Recognizes R-Ras; human R-Ras is a 218 amino acid protein.

Alternate Names

Oncogene RRAS, p23, ras-related protein R-Ras, related RAS viral (r-ras) oncogene homolog

Gene Symbol

RRAS

Additional RRAS Products

Product Documents for RRAS Antibody (OTI2B7)

Certificate of Analysis

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Product Specific Notices for RRAS Antibody (OTI2B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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