SAG

Tocris Bioscience | Catalog # 4366

Potent Smoothened receptor agonist; activates the Hedgehog signaling pathway
Tocris Bioscience

Key Product Details

Description

Potent Smoothened receptor agonist; activates the Hedgehog signaling pathway
View all Smoothened Receptor Agonists »

Product Description

SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons.

SAG dihydrochloride (Cat. No. 6390) also available.

Product Specifications for SAG

Molecular Weight

490.06

Formula

C28H28ClN3OS

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide

CAS Number

912545-86-9

PubChem ID

5284330

InChI Key

VFSUUTYAEQOIMW-UHFFFAOYSA-N

SMILES

ClC2=C(C(N([C@H]4CC[C@H](NC)CC4)CC3=CC=CC(C5=CC=NC=C5)=C3)=O)SC1=CC=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 49.01 100

Preparing Stock Solutions for SAG

The following data is based on the product molecular weight 490.06.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.04 mL 10.20 mL 20.41 mL
5 mM 0.41 mL 2.04 mL 4.08 mL
10 mM 0.20 mL 1.02 mL 2.04 mL
50 mM 0.04 mL 0.20 mL 0.41 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 70 publications citing the usage of this product.

Product Documents for SAG

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SAG

For research use only

Customer Reviews for SAG (2)

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  • Promoted the formation of cilia
    Name: Kishor Pant
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HepG2, and Huh,
    Verified Customer | Posted 02/10/2023
    We use 5-20 nm concentration for 24-48 hours.
    SAG consistently produced positive results in our experiments; it promoted the formation of cilia and reduced the proliferation of cancer cells.
    SAG 4366
  • Usage Information
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Verified Customer | Posted 07/04/2020
    From Day 2 to day 9 to direct the cells toward ventral diencephalon with regular media change every 2 days.
    iPSC-derived hypothalamic neuron differentiation activation by Smoothened agonist SAG 1 μM and purmorphamine 1 μM.
    SAG 4366

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