SARS-CoV-2 nsp1 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-15978
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Key Product Details
Species Reactivity
SARS-CoV-2
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of coronavirus NSP1 (YP_009742608.1). MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 nsp1 Antibody - Azide and BSA Free
Western Blot: SARS-CoV-2 nsp1 AntibodyAzide and BSA Free [NBP3-15978]
Western Blot: SARS-CoV-2 nsp1 Antibody [NBP3-15978] - Western blot analysis of extracts of normal 293T cells 293T transfected with NSP1 Protein, using SARS-CoV-2 nsp1 antibody (NBP3-15978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunoprecipitation: SARS-CoV-2 nsp1 Antibody - Azide and BSA Free [NBP3-15978]
Immunoprecipitation: SARS-CoV-2 nsp1 Antibody [NBP3-15978] - Immunoprecipitation analysis of 600ug extracts of 293T cells using 3ug SARS-CoV-2 nsp1 antibody (NBP3-15978). Western blot was performed from the immunoprecipitate using SARS-CoV-2 nsp1 antibody (NBP3-15978) at a dilition of 1:1000.Applications for SARS-CoV-2 nsp1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 nsp1
Alternate Names
Non-structural protein 1, nsp1, ORF1a polyprotein, ORF1ab polyprotein, pp1a, Replicase polyprotein 1a
Gene Symbol
ORF1ab
Additional SARS-CoV-2 nsp1 Products
Product Documents for SARS-CoV-2 nsp1 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SARS-CoV-2 nsp1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.govCustomer Reviews for SARS-CoV-2 nsp1 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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