SC 514

Tocris Bioscience | Catalog # 3318

IKKβ inhibitor; attenuates NF-κB-induced gene expression
Tocris Bioscience
Discontinued Product
3318 has been discontinued. View all IkB Kinase Inhibitors products.

Key Product Details

Description

IKKβ inhibitor; attenuates NF-κB-induced gene expression
View all IkB Kinase Inhibitors »

Product Description

SC 514 is an orally active, ATP-competitive IKKβ inhibitor (IC50 = 3 - 12 μM) that displays > 10-fold selectivity over 28 other kinases including JNK, p38, MK2 and ERK. Attenuates NF-κB-induced gene expression of IL-6, IL-8 and COX-2 in synovial fibroblasts (IC50 values are 20, 20 and 8 μM respectively). Reduces iNOS induction and exhibits anti-inflammatory activity in vivo.

Licensing Information

Sold for research purposes under agreement from AstraZeneca

Product Specifications for SC 514

Molecular Weight

224.30

Formula

C9H8N2OS2

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

4-Amino-[2',3'-bithiophene]-5-carboxamide

CAS Number

354812-17-2

PubChem ID

2807869

InChI Key

BMUACLADCKCNKZ-UHFFFAOYSA-N

SMILES

NC1=C(C(N)=O)SC(C2=CSC=C2)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 22.43 100
Ethanol 2.24 10

Preparing Stock Solutions for SC 514

The following data is based on the product molecular weight 224.30.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.46 mL 22.29 mL 44.58 mL
5 mM 0.89 mL 4.46 mL 8.92 mL
10 mM 0.45 mL 2.23 mL 4.46 mL
50 mM 0.09 mL 0.45 mL 0.89 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 20 publications citing the usage of this product.

Product Documents for SC 514

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SC 514

For research use only

Customer Reviews for SC 514 (1)

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  • SC 514 inhibits LPS-mediated inflammation
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Macrophages
    Verified Customer | Posted 12/11/2018
    RAW macrophages were incubated with 1 mM SC 514 for 30 min prior to addition of 200 ng/ml LPS for 8 h to follow TNFa mRNA expression. LPS elevated TNFa mRNA levels that was significantly inhibited by pretreatment of cells with SC 514.
    SC 514 3318

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