sCy5DA

Tocris Bioscience | Catalog # 7834

FDAA for super-resolution microscopy of bacteria
Tocris Bioscience

Key Product Details

Description

FDAA for super-resolution microscopy of bacteria

Wavelength

Excitation = 646 nm, Emission = 665 nm
View all Fluorescent Probes for Imaging Bacteria »

Product Description

sCy5DA is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans in live bacteria. It is efficiently incorporated into the peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into gram-negative C. crescentus. sCy5DA labelled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/665 nm.

Scientific Data Images for sCy5DA

Vibrio cholerae cells stained with sCy5DA

Single molecule localization microscopy (SMLM) image of Vibrio cholerae cells labeled with 500 μM sCy5DA

Bacillus subtilis cells stained with sCy5DA

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA

Mycobacterium smegmatis cells stained with sCy5DA

Single molecule localization microscopy (SMLM) image of Mycobacterium smegmatis cells labeled with 100 μM sCy5DA

Staphylococcus aureus cells stained with sCy5DA

Single molecule localization microscopy (SMLM) image of Staphylococcus aureus labeled with 100 μM sCy5DA

Optical Data

sCy5DA Dye Spectra
Emission Color Red
λabs 646 nm
λem 665 nm
Extinction Coefficient (ε) 197,240 M-1cm-1
Cell Permeable Yes
Application STORM

Product Specifications for sCy5DA

Molecular Weight

728.88

Formula

C35H44N4O9S2

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(R)-2-Ammonio-3-(6-((E)-3,3-dimethyl-5-sulfo-2-((2E,4E)-5-(1,3,3-trimethyl-5-sulfonato-3H-indol-1-ium-2-yl)penta-2,4-dien-1-ylidene)indolin-1-yl)hexanamido)propanoate

InChI Key

ZSTSSXALPFZNBO-HHHXNRCGSA-N

SMILES

CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(NC[C@H](C([O-])=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.29 10

Preparing Stock Solutions for sCy5DA

The following data is based on the product molecular weight 728.88.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 13.72 mL 68.60 mL 137.20 mL
0.5 mM 2.74 mL 13.72 mL 27.44 mL
1 mM 1.37 mL 6.86 mL 13.72 mL
5 mM 0.27 mL 1.37 mL 2.74 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for sCy5DA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for sCy5DA

For research use only

Customer Reviews for sCy5DA (1)

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  • Obligate anaerobe PG probing
    Name: Benjamin Lewis
    Species: Bacteria (Porphyromonas gingivalis)
    Assay Type: In Vitro
    Verified Customer | Posted 04/24/2025
    Labeled log phase, bacteriostatic, and heat-killed cultures of P.gingivalis with sCy5DA (0.2 mM) to demonstrate growth status and viability.
    sCy5DA 7834

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