Recombinant Human Serum Amyloid A Protein

Novus Biologicals | Catalog # NBP2-61366

Novus Biologicals
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Key Product Details

Source

E. coli
View all Serum Amyloid A Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 104 amino acids corresponding to Recombinant Human Apolipoprotein-Serum Amyloid A

Source: E. coli

Accession #:: AAA64799.1

Amino Acid Sequence: RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISN ARENIQRFFG RGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY

There are two amino acid difference between the sequence of NBP2-61366 and the reference sequence Accession: AAA64799.1: presence of an N-terminal methionine and substitution of asparagine for aspartic acid at position 60 and arginine for histidine at position 71 (the later two substituted residues are present in apoSAA2beta).

Purity

>98%, by SDS-PAGE and HPLC

Predicted Molecular Mass

11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by its ability to down-regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 uM.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Serum Amyloid A Protein

Recombinant Human Serum Amyloid A1 Protein SDS-PAGE: Recombinant Human Serum Amyloid A Protein [NBP2-61366] -

SDS-PAGE: Recombinant Human Serum Amyloid A Protein [NBP2-61366] -

SDS-PAGE of Recombinant Human Serum Amyloid A Protein.

Formulation, Preparation, and Storage

NBP2-61366
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 9.0, 150 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serum Amyloid A

The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a quot houekeeping quot role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis. Amyloidosis is a heterogeneous group of disorders characterized by extracellular deposition of abnormal protein fibrils, which are derived from different proteins. Almost all patients with amyloidosis have some level of renal disorders. The immunoperoxidase technique has the advantage of being able to detect amyloid in formalin-fixed paraffin embedded tissues and is a very specific method for identifying amyloid fibril proteins. Small and minute amounts of amyloid can be detected with both Amyloid P (ab688) and Amyloid A antibodies, and thus could allow earlier treatment before organ damage has occurred.

Gene Symbol

SAA1

Additional Serum Amyloid A Products

Product Documents for Recombinant Human Serum Amyloid A Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Serum Amyloid A Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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