Simple Plex Cytokine Release Panel 2

R&D Systems | Catalog # ST01C-PS-009470

Simple Plex 32x4 panel containing IL-1b,IL-6 3rd gen,IL-8,TNF-a 2nd gen with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).
R&D Systems
Loading...

Key Product Details

Features

The Simple Plex Human Multianalyte Assay is for the detection of human IL-1b, IL-6 3rd gen, IL-18, and TNF-a 2nd gen in plasma/serum samples.

 
Sample Type & Volume Required Per Well Plasma/Serum (25 uL)
Sensitivity IL-1b 0.064 pg/mL; IL-6 3rd gen 0.11 pg/mL; IL-8 0.08 pg/mL; TNF-a 2nd gen 0.3 pg/mL
Assay Range IL-1b 0.4 - 1,530 pg/mL; IL-6 3rd gen 0.28 - 2,652 pg/mL; IL-8 0.19 - 1,804 pg/mL; TNF-a 2nd gen 0.3 - 1,160

Species

Human

Applications

Simple Plex Automated Immunoassay

Product Summary for Simple Plex Cytokine Release Panel 2

Product Specifications

Reactivity Human
Specificity Natural and recombinant human IL-1b, IL-6 3rd gen, IL-18, and TNF-a 2nd gen.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IL-1b n 16 16 16 16
Mean (pg/mL) 8.66 446 8.42 410
Standard Deviation 0.427 16.5 0.342 23.3
CV (%) 4.9 3.7 4.0 5.7
IL-6 3rd gen n 16 16 40 40
Mean (pg/mL) 23.1 876 23.6 1008
Standard Deviation 0.899 72.1 1.82 77.7
CV (%) 3.9 8.2 7.7 7.7
IL-8 n 16 16 10 10
Mean (pg/mL) 28.7 1362 27.3 1463
Standard Deviation 1.41 89.8 1.41 125
CV (%) 4.9 6.6 5.2 8.6
TNF-a 2nd gen n 16 16 36 35
Mean (pg/mL) 6.99 383 7.81 408
Standard Deviation 0.345 20.6 0.662 36.9
CV (%) 4.9 5.4 8.5 9.0

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
IL-1b EDTA Plasma (n=4) 106 97-120
Heparin Plasma (n=4) 99 92-112
Serum (n=4) 105 96-112
IL-6 3rd gen EDTA Plasma (n=4) 92 86-102
Heparin Plasma (n=4) 87 85-90
Serum (n=4) 94 90-99
IL-8 EDTA Plasma (n=5) 89 75-102
Heparin Plasma (n=5) 95 88-102
Serum (n=3) 92 88-97
TNF alpha 2nd Gen EDTA Plasma (n=4) 97 89-105
Heparin Plasma (n=4) 89 77-96
Serum (n=4) 99 92-108
Loading...

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Simple Plex Cytokine Release Panel 2

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Cytokine Release Panel 2

For research use only

Citations for Simple Plex Cytokine Release Panel 2

View all citations for the Simple Plex platform on Bio-Techne.com.

Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Cytokine Release Panel 2

There are currently no reviews for this product. Be the first to review Simple Plex Cytokine Release Panel 2 and earn rewards!

Have you used Simple Plex Cytokine Release Panel 2?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card