Simple Plex Discovery beta-Defensin 2 Assay New

Catalog #: SPCKB-PS-014655 Datasheet / COA / SDS

Simple Plex beta-Defensin 2 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

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SPCKA-PS-014655
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Control Products Available

897693: Simple Plex Discovery Control for Human beta-Defensin 2

Simple Plex Discovery Human beta -Defensin 2 Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery beta-Defensin 2 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

22.5 pg/mL

Assay Range

85.2 - 52,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human beta -Defensin 2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human beta -Defensin 2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one beta -Defensin 2 assay

Inter-Assay Precision (Precision between assays) Replicates of each beta -Defensin 2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human beta -Defensin 2

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	50	52
Mean (pg/mL)	471	22753	384	20887
Standard Deviation	18.3	655	44	1988
CV%	3.9	2.9	11.4	9.5

Recovery

Recovery for the human beta -Defensin 2 assays was evaluated at three different spiked concentrations of beta -Defensin 2 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	91	87-94
EDTA Plasma (n=2)	90	78-102
Heparin Plasma (n=2)	100	82-117
Serum (n=2)	105	91-120

Linearity

Samples containing and/or spiked with high concentrations of beta -Defensin 2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human beta -Defensin 2 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human beta -Defensin 2 Assay. Assay range is 85.2-52,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta-Defensin 2

Defensins are members of a family of microbicidal and cytotoxic peptides produced by neutrophils. They are classified into alpha-, beta-, and theta-defensins based on their sizes and intramolecular disulfide bond patterns. Human beta-Defensin 2 (BD-2; aa 24 - 64) also known as defensin beta 2 (DEFB-2), is a secreted, 41 amino acid residue anti-microbial protein belonging to the defensin family. Mature defensins are cationic proteins with three intramolecular disulfides and a characteristic triple-stranded beta-sheet. Human beta-Defensin 2 expression is induced by inflammatory signals in multiple tissues, including airway epithelial cells and keratinocytes. At the amino acid sequence level, human beta-Defensin 2 shares 98% and 51% homology with the proteins from rhesus monkey and rat, respectively.

Long Name:

Defensin 2 beta

Entrez Gene IDs:

1673 (Human)

Alternate Names:

BD-2; betaDefensin 2; beta-Defensin 2; DEFB2; DEFB-2; DEFB2DEFB102; DEFB4A; DEFB4B; DEFB4beta-defensin 4A; Defensin, beta 2Skin-antimicrobial peptide 1; defensin, beta 4; defensin, beta 4A; HBD-2; SAP-1; SAP1BD-2