Simple Plex Discovery Human CG beta Assay New

Catalog #: SPCKB-PS-012702 Datasheet / COA / SDS

Simple Plex Human CG beta assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012702
SPCKA-PS-012702
SPCKC-PS-012702

Control Products Available

897214: Simple Plex Discovery Control for Human CG beta

Simple Plex Discovery Human CG beta Ella Assay

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All Chorionic Gonadotropin beta Chain (HCG beta) Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human CG beta Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.3 pg/mL

Assay Range

1.3 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human CG beta.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Chorionic Gonadotropin Beta Chain (CG beta ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	25
Mean (pg/mL)	17.3	664	15	694
Standard Deviation	0.763	19.3	1.69	35.8
CV%	4.4	2.9	11.3	5.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	100-103
EDTA Plasma (n=2)	109	100-117
Heparin Plasma (n=2)	108	103-113
Serum (n=2)	116	115-116

Linearity

Samples containing and/or spiked with high concentrations of human CG beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human CG beta Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chorionic Gonadotropin beta Chain (HCG beta)

HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.

Long Name:

Chorionic Gonadotropin beta Chain

Entrez Gene IDs:

1082 (Human)

Alternate Names:

CGB; CGB3; CGB3choriogonadotropin subunit beta; CGB5; CGB7; CGB8; CG-Beta; Choriogonadotropin Subunit Beta; Chorionic gonadotrophin chain beta; chorionic gonadotropin beta 3 subunit; Chorionic Gonadotropin beta Chain; chorionic gonadotropin beta subunit; chorionic gonadotropin, beta polypeptide; hCGB