Simple Plex Discovery Human Coagulation Factor III Assay

R&D Systems | Catalog # SPCKB-PS-012018

Simple Plex Human Coagulation Factor III assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

Customize My Cartridge on Bio-Techne

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com

Customize My Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.25 pg/mL

Assay Range

1.19-4548 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Coagulation Factor III Assay Summary

Simple Plex Discovery assay is for the detection of human Coagulation Factor III in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Coagulation Factor III/Tissue Factor Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
了解更多

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Coagulation Factor III. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 31 31
Mean (pg/mL) 31.4 1698 33.8 1625
Standard Deviation 3.97 68.3 3.62 132
CV% 7.2 4.0 10.7 8.1

Recovery for Simple Plex Discovery Human Coagulation Factor III Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 101 100-101
EDTA Plasma (n=2) 110 108-112
Heparin Plasma (n=2) 111 106-116
Serum (n=2) 96 93-99

Linearity

Samples containing and/or spiked with high concentrations of recombinant human Coagulation Factor III were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Coagulation Factor III/Tissue Factor Ella Assay

Scientific Data Images for Simple Plex Discovery Human Coagulation Factor III Assay

Simple Plex Discovery Human Coagulation Factor III/Tissue Factor Ella Assay

Simple Plex Discovery Human Coagulation Factor III/Tissue Factor Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Gene Symbol

F3

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Simple Plex Discovery Human Coagulation Factor III Assay

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Coagulation Factor III Assay

For research use only

Citations for Simple Plex Discovery Human Coagulation Factor III Assay

View all citations for the Simple Plex platform on Bio-Techne.com.

Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Discovery Human Coagulation Factor III Assay

There are currently no reviews for this product. Be the first to review Simple Plex Discovery Human Coagulation Factor III Assay and earn rewards!

Have you used Simple Plex Discovery Human Coagulation Factor III Assay?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card