Simple Plex Discovery Human DDL1 Assay New

Catalog #: SPCKB-PS-012304 Datasheet / COA / SDS

Simple Plex Human DDL1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012304
SPCKA-PS-012304
SPCKC-PS-012304

Control Products Available

897192: Simple Plex Discovery Control for Human DLL1

Simple Plex Discovery Human DLL1 Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human DDL1 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.69 pg/mL

Assay Range

2.4 - 9,004 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human DLL1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Delta-like Protein 1 (DLL1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	34
Mean (pg/mL)	66.6	3137	70.2	3153
Standard Deviation	2.09	85.9	7.05	269
CV%	3.1	2.7	10.1	8.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	120	118-121

Linearity

Samples containing and/or spiked with high concentrations of human DLL1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human DLL1 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DLL1

DLL1 (Delta-like protein 1) is a transmembrane glycoprotein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. It plays a role in central nervous system morphogenesis, intervertebral joint formation, inner ear hair cell development and patterning, and arteriogenesis. It regulates the homeostasis and differentiation of neural, pancreatic, and myogenic progenitor cells, T and B lymphocytes. DLL1 is overexpressed in a number of cancers and contributes to tumorigenesis. The extracellular region of DLL1 can be shed from the cell surface, and the intracellular domain can be cleaved by gamma-secretase to translocate to the nucleus and regulate gene transcription.

Long Name:

Delta-like 1

Entrez Gene IDs:

28514 (Human); 13388 (Mouse); 84010 (Rat)

Alternate Names:

delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1