Simple Plex Discovery Human IL-16 Assay New

Catalog #: SPCKB-PS-012153 Datasheet / COA / SDS

Simple Plex Human IL-16 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

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Control Products Available

897185: Simple Plex Discovery Control for Human IL-16

Simple Plex Discovery Human IL-16 Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human IL-16 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.18 pg/mL

Assay Range

0.3 - 3,200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-16.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Interleukin 16 (IL-16) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	22	22
Mean (pg/mL)	28.7	1349	23.1	1130
Standard Deviation	0.926	72	1.91	85.2
CV%	3.2	5.3	8.2	7.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	107	106-109

Linearity

Samples containing and/or spiked with high concentrations of human IL-16 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human IL-16 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-16

Interleukin 16 (IL-16), also named lymphocyte chemoattractant factor (LCF), is a 14-17 kDa single chain non-glycosylated polypeptide that was originally identified as a CD8+ T cell-derived chemoattractant for CD4+ cells. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. In addition to its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro.

Long Name:

Interleukin 16

Entrez Gene IDs:

3603 (Human); 16170 (Mouse)

Alternate Names:

FLJ16806; FLJ42735; FLJ44234; HsT19289; IL16; IL-16; interleukin 16 (lymphocyte chemoattractant factor); LCF; LCFprIL-16; lymphocyte chemoattractant factor; neuronal interleukin 16; NIL16; PRIL16; prointerleukin 16; pro-interleukin-16