Simple Plex Discovery Human MBL Assay New

Catalog #: SPCKB-PS-012016 Datasheet / COA / SDS

Simple Plex Human MBL assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012016
SPCKA-PS-012016
SPCKC-PS-012016

Control Products Available

897165: Simple Plex Discovery Control for Human MBL

Simple Plex Discovery Human MBL Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human MBL Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

1.51 pg/mL

Assay Range

3.7 - 14,130 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MBL.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Mannan-Binding Lectin (MBL) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	24
Mean (pg/mL)	87.3	4484	89.4	4324
Standard Deviation	1.6	159	4.37	206
CV%	2.6	3.5	4.9	4.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	90	88-93

Linearity

Samples containing and/or spiked with high concentrations of recombinant human MBL were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human MBL Ella Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MBL

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which bind an array of carbohydrate patterns on pathogen surfaces. Collectin family members share several common structural features including a N-terminal, cysteine-rich domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal, C-type (Ca2+-dependent ) lectin or carbohydrate recognition domain. MBL homotrimerizes to form a structural unit joined by N-terminal disulfide bonds. These homotrimers further associate into oligomeric structures of up to six units.

Long Name:

Mannose Binding Lectin

Entrez Gene IDs:

4153 (Human)

Alternate Names:

COLEC1; COLEC1collectin-1; Collectin-1; HSMBPC; Mannan-binding protein; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble; Mannose-binding lectin; mannose-binding protein C; MBL; MBL2; MBL2D; MBLmannan-binding lectin; MBP; MBP1; MBP1mannose-binding lectin 2, soluble (opsonic defect); MBP-C; MGC116832; MGC116833