Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay New

Catalog #: SPCKB-PS-014625 Datasheet / COA / SDS

Simple Plex Human OX40 Ligand assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-014625
SPCKA-PS-014625
SPCKC-PS-014625

Control Products Available

897967: Simple Plex Discovery Control for Human OX40 Ligand

Simple Plex Discovery Human OX40 Ligand Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.58 pg/mL

Assay Range

3.1 - 12,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human OX40 Ligand.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human OX40 Ligand/TNFSF4 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one OX40 Ligand assay

Inter-Assay Precision (Precision between assays) Replicates of each OX40 Ligand control were tested in multiple assays performed by at least three technicians using two lots of reagents for human OX40 Ligand

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	33
Mean (pg/mL)	156	7307	92.5	3910
Standard Deviation	11.9	475	8	335
CV%	7.6	6.5	8.7	8.6

Recovery

Recovery for the human OX40 Ligand assays was evaluated at three different spiked concentrations of OX40 Ligand protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	104	91-117
EDTA Plasma (n=2)	85	74-95
Heparin Plasma (n=2)	100	95-104
Serum (n=2)	90	86-95

Linearity

Samples containing and/or spiked with high concentrations of OX40 Ligand were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human OX40 Ligand Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human OX40 Ligand Assay. Assay range is 3.14-12,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a member of the TNF superfamily. Human OX40L shares 46% amino acid sequence identity with its mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similar to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily, which is expressed predominantly on activated CD4+ T cells.

Entrez Gene IDs:

7292 (Human); 22164 (Mouse); 89814 (Rat)

Alternate Names:

CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1