Simple Plex Discovery Human Peptide YY Cartridge

R&D Systems | Catalog # SPCKB-PS-016664

Simple Plex Discovery Human Peptide YY assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

15.9 pg/mL

Assay Range

34.4-3360 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Peptide YY Cartridge Summary

Simple Plex Discovery assay is for the detection of human Peptide YY in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Peptide YY Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
了解更多

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Recombinant Peptide YY (3-36) and recombinant Peptide YY (1-36). 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Peptide YY assay.

Inter-Assay Precision (Precision between assays) Replicates of each Peptide YY control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Peptide YY.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 17 28
Mean (pg/mL) 68.0 1207 64.6 1151
Standard Deviation 2.74 17.6 5.67 51.9
CV% 4.0 1.5 8.8 4.5

Recovery for Simple Plex Discovery Human Peptide YY Cartridge

Recovery for the human Peptide YY assays was evaluated at three different spiked concentrations of Peptide YY protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 103 101-104
EDTA Plasma (n=2) 106 101-111
Heparin Plasma (n=2) 107 104-110
Serum (n=2) 106 103-108

Linearity

Samples containing and/or spiked with high concentrations of Peptide YY were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Peptide YY Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Peptide YY Cartridge

Simple Plex Discovery Human Peptide YY Assay Standard Curve

Simple Plex Discovery Human Peptide YY Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Peptide YY Assay. Assay range is 34.4-3360 pg/mL.
Simple Plex Discovery Human Peptide YY Assay Endogenous Levels

Simple Plex Discovery Human Peptide YY Assay Endogenous Levels

Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Peptide YY

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility

Alternate Names

Peptide-YY, PYY, PYY-I, PYY1

Entrez Gene IDs

5697 (Human); 217212 (Mouse); 287730 (E. faecalis)

Gene Symbol

PYY

Additional Peptide YY Products

Product Documents for Simple Plex Discovery Human Peptide YY Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Peptide YY Cartridge

For research use only

Citations for Simple Plex Discovery Human Peptide YY Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Discovery Human Peptide YY Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Discovery Human Peptide YY Cartridge and earn rewards!

Have you used Simple Plex Discovery Human Peptide YY Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card