Simple Plex Discovery Human Serpin F1/PEDF Assay New

Catalog #: SPCKB-PS-015287 Datasheet / COA / SDS

Simple Plex Discovery Human Serpin F1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-015287
SPCKA-PS-015287
SPCKC-PS-015287

Control Products Available

897696: Simple Plex Discovery Control for Human Serpin F1/PEDF

Simple Plex Human Serpin F1/PEDF Assay Standard Curve

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Serpin F1/PEDF Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma ( uL)

Sensitivity

4.21 pg/mL

Assay Range

7.9 - 12,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Serpin F1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Serpin F1/PEDF in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Recovery

Recovery for the human Serpin F1/PEDF assays was evaluated at three different spiked concentrations of Serpin F1/PEDF protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	112	111-113

Linearity

Samples containing and/or spiked with high concentrations of Serpin F1/PEDF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Human Serpin F1/PEDF Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Serpin F1/PEDF Assay. Assay range is 7.86-12,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin F1/PEDF

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Long Name:

Serpine F1/Pigment Epithelium-derived Factor

Entrez Gene IDs:

5176 (Human); 20317 (Mouse); 287526 (Rat)

Alternate Names:

Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1