Simple Plex Discovery Human Spinesin Assay New

Catalog #: SPCKB-PS-012120 Datasheet / COA / SDS

Simple Plex Human Spinesin assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012120
SPCKA-PS-012120
SPCKC-PS-012120

Control Products Available

897162: Simple Plex Discovery Control for Spinesin

Simple Plex Discovery Human Spinesin Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Spinesin Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.66 pg/mL

Assay Range

2.6 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Spinesin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Spinesin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	26	27
Mean (pg/mL)	31.4	1447	29.8	1360
Standard Deviation	1.11	53.3	3.31	88.8
CV%	3.5	3.7	11.1	6.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	104	102-105
EDTA Plasma (n=2)	77	76-79
Heparin Plasma (n=2)	83	82-84
Serum (n=2)	87	81-92

Linearity

Samples containing and/or spiked with high concentrations of human Spinesin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human Spinesin Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Spinesin

Spinesin is a type II transmembrane serine protease (TTSP). TTSPs have emerged as an important group of cell surface proteases with key roles in converting protein precursors into mature and functional molecules. All TTSPs are synthesized as single-chain precursors and processed into disulfide bond-linked dimers.

Entrez Gene IDs:

80975 (Human); 80893 (Mouse)

Alternate Names:

EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; MGC141886; MGC148044; Spinesin; TMPRSS5; transmembrane protease serine 5; transmembrane protease, serine 5