Simple Plex Human alpha-Synuclein Cartridge

Catalog #: SPCKC-PS-011989 Datasheet / COA / SDS

Simple Plex Human alpha-Synuclein assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKC-PS-011989
SPCKA-PS-011989

4 Images

Product Details

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human alpha-Synuclein Cartridge Summary

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

0.82 pg/mL

Assay Range

2.6 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human alpha -Synuclein.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human alpha-Synuclein ( alpha -Synuclein) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	54	54
Mean (pg/mL)	22.2	862	16.6	745
Standard Deviation	0.974	60.5	1.45	79.2
CV%	4.4	7	8.8	10.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	115	115-116
Cerebrospinal Fluid (n=4)	115	109-118

Linearity

Samples containing and/or spiked with high concentrations of recombinant human alpha -Synuclein were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

View Larger

Human alpha-Synuclein Ella Assay Standard Curve

N/A

View Larger

Human alpha-Synuclein Ella Assay Endogenous Levels Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha-Synuclein

The synuclein family includes alpha-Synuclein, beta-Synuclein, gamma-Synuclein, and synoretin. These small, highly conserved proteins are localized predominantly in the brain, concentrated in presynaptic nerve terminals. Alpha-Synuclein is normally a soluble protein. Abnormal alpha-synuclein forms filamentous inclusions (Lewy bodies and Lewy neurites) in several neurodegenerative diseases, including Parkinson's disease (PD), dementia with Lewy bodies, and Alzheimer's disease. Beta-Synuclein and gamma-Synuclein (synoretin) associate with alpha-synuclein and inhibit its aggregation.

Entrez Gene IDs:

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names:

alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein