Simple Plex Human E-Cadherin Cartridge

Catalog #: SPCKB-PS-000208 Datasheet / COA / SDS

Simple Plex Human E-Cadherin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000208
SPCKA-PS-000208
SPCKC-PS-000208

Control Products Available

898083: Simple Plex Control for Human E-Cadherin

Ella Automated ELISA Platform and Simple Plex Human E-Cadherin Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human E-Cadherin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)

Sensitivity

13.3 pg/mL

Assay Range

9.9 - 15,140 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human E-Cadherin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human E-Cadherin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one E-Cadherin assay

Inter-Assay Precision (Precision between assays) Replicates of each E-Cadherin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human E-Cadherin

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	23
Mean (pg/mL)	75	3596	67.7	3841
Standard Deviation	1.79	134	6.83	399
CV%	2.4	3.7	10.1	10.4

Recovery

Recovery for the human E-Cadherin assays was evaluated at three different spiked concentrations of E-Cadherin protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	100	94-107

Linearity

Samples containing and/or spiked with high concentrations of human E-Cadherin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human E-Cadherin Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human E-Cadherin Assay. Assay range is 9.92-15140 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2⁺-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Entrez Gene IDs:

999 (Human); 12550 (Mouse); 83502 (Rat)

Alternate Names:

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin