Simple Plex Human HVEM/TNFRSF14 Cartridge

Catalog #: SPCKB-PS-003550 Datasheet / COA / SDS

Simple Plex Human HVEM/TNFRSF14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-003550
SPCKA-PS-003550
SPCKC-PS-003550

Control Products Available

898774: Simple Plex Control for Human HVEM/TNFRSF14

Simple Plex Human HVEM Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human HVEM/TNFRSF14 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

0.12 pg/mL

Assay Range

1.7 - 16,240 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human HVEM.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Herpesvirus Entry Mediator (HVEM) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one HVEM assay

Inter-Assay Precision (Precision between assays) Replicates of each HVEM control were tested in multiple assays performed by at least three technicians using two lots of reagents for human HVEM

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	34
Mean (µL)	96.8	4590	95.2	4678
Standard Deviation	8.14	348	9.93	365
CV%	8.4	7.6	10.4	7.8

Recovery

Recovery was not necessary as human HVEM shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human HVEM were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Human HVEM Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human HVEM Assay. Assay range is 1.70-16,240 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HVEM/TNFRSF14

HVEM/TNFRSF14 (herpesvirus entry mediator) is a transmembrane protein that is most highly expressed on naïve, memory, and regulatory T cells but declines during T cell activation. It is present at low levels on most resting leukocytes. HVEM is a receptor for BTLA, CD160, and LIGHT. A cis interaction between HVEM and BTLA promotes cell survival, while trans interaction promotes a state of lymphocyte inactivity. LIGHT is expressed following T cell activation and competes with BTLA for HVEM binding, leading to a proinflammatory response. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding. Human, but not mouse, HVEM can also bind lymphotoxin-alpha.

Long Name:

Herpesvirus Entry Mediator

Entrez Gene IDs:

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Alternate Names:

ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2