Simple Plex Human IL-1ra/IL-1F3 Cartridge

R&D Systems | Catalog # SPCKB-PS-000323

Simple Plex Cartridge Kit for quantitation of IL-1ra. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.3 pg/mL

Assay Range

7.37-4500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human IL-1ra/IL-1F3 Cartridge Summary

Simple Plex assay for the detection of human IL-1ra in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-1ra/IL-1F3 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-!ra.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 39 35
Mean (pg/mL) 25.3 1310 27.2 1390
Standard Deviation 1.47 100 2.00 69.8
CV% 5.8 7.7 7.4 5.0

Recovery for Simple Plex Human IL-1ra/IL-1F3 Cartridge

Recovery was not necessary as IL-1ra shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human IL-1ra were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human IL-1ra Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human IL-1ra/IL-1F3 Cartridge

Human IL-1ra Simple Plex Assay Standard Curve

Human IL-1ra Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1ra/IL-1F3

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Entrez Gene IDs

3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine); 100034236 (Equine)

Gene Symbol

IL1RN

Additional IL-1ra/IL-1F3 Products

Product Documents for Simple Plex Human IL-1ra/IL-1F3 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human IL-1ra/IL-1F3 Cartridge

For research use only

Citations for Simple Plex Human IL-1ra/IL-1F3 Cartridge

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