Simple Plex Human TIM-3 Cartridge
R&D Systems | Catalog # SPCKB-PS-001049
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human TIM-3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
精度
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TIM-3 assay.
Inter-Assay Precision (Precision between assays) Replicates of each TIM-3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TIM-3.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 21 | 22 |
| Mean (pg/mL) | 34.0 | 1645 | 40.2 | 1964 |
| Standard Deviation | 2.76 | 125 | 4.40 | 196 |
| CV% | 8.0 | 8.0 | 11.0 | 10.0 |
Recovery for Simple Plex Human TIM-3 Cartridge
Recovery was not necessary as human TIM-3 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human TIM-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human TIM-3 Cartridge
Simple Plex Human TIM-3 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TIM-3 Assay. Assay range is 2.04-7780 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: TIM-3
TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2 is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. It is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). TIM-3 ligation by Galectin-9 attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. Dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. TIM-3 also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional TIM-3 Products
Product Documents for Simple Plex Human TIM-3 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human TIM-3 Cartridge
For research use only
Citations for Simple Plex Human TIM-3 Cartridge
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