Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

R&D Systems | Catalog # SPCKB-MP-000583

Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (500 nL), Mouse Serum (25 µL), Mouse EDTA Plasma (25 µL), Mouse Heparin Plasma (25 µL)

Sensitivity

0.06 pg/mL

Assay Range

0.92-1412 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge Summary

Simple Plex assay for the detection of mouse MIP-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CXCL2/GRO beta/MIP-2/CINC-3 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse MIP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 91 92
Mean (pg/mL) 10.9 475 10.3 463
Standard Deviation 0.994 40.8 1.20 39.3
CV% 9.1 8.6 11.6 8.5

Recovery for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Mouse EDTA Plasma (n=4) 106 100-115
Mouse Heparin Plasma (n=4) 107 100-116
Mouse Serum (n=4) 104 94-113

Linearity

Samples containing and/or spiked with high concentrations of mouse MIP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Mouse CXCL2/MIP-2 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

Mouse CXCL2/MIP-2 Simple Plex Assay Standard Curve

Mouse CXCL2/MIP-2 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL2/GRO beta/MIP-2/CINC-3

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2

Entrez Gene IDs

2920 (Human); 20310 (Mouse); 114105 (Rat)

Gene Symbol

CXCL2

Additional CXCL2/GRO beta/MIP-2/CINC-3 Products

Product Documents for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

For research use only

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