Simple Plex Mouse NF-L Cartridge

R&D Systems | Catalog # SPCKB-MP-003168

Simple Plex Mouse NFL assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with MP (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

1.09 pg/mL

Assay Range

2.7-10290 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Mouse NF-L Cartridge Summary

Simple Plex assay is for the detection of mouse Neurofilament Light (NF-L) in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all NF-L Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
了解更多

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant NF-L.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 48 48
Mean (pg/mL) 76.6 3309 80.5 3476
Standard Deviation 3.64 154 8.35 267
CV% 4.8 4.7 10.4 7.7

Recovery for Simple Plex Mouse NF-L Cartridge

Recovery was not necessary as mouse NF-L shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of mouse NF-L were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Mouse NF-L Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Mouse NF-L Cartridge

Mouse NF-L Ella Assay Standard Curve

Mouse NF-L Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-L

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for Simple Plex Mouse NF-L Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Mouse NF-L Cartridge

For research use only

Citations for Simple Plex Mouse NF-L Cartridge

View all citations for the Simple Plex platform on Bio-Techne.com.

Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Mouse NF-L Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Mouse NF-L Cartridge and earn rewards!

Have you used Simple Plex Mouse NF-L Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card