Simple Plex Mouse/Rat TREM-2 Cartridge

R&D Systems | Catalog # SPCKB-MP-008555

Simple Plex Mouse/Rat TREM-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

0.036 pg/mL

Assay Range

5.24-8000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Mouse/Rat TREM-2 Cartridge Summary

Simple Plex assay is for the detection of mouse and rat Triggering Receptor Expressed on Myeloid cells 2 (TREM-2) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD09. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TREM2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse, Rat

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 74 74
Mean (pg/mL) 64.8 2887 60.2 2812
Standard Deviation 4.9 189 5.42 283
CV% 7.5 6.5 9.0 10.1

Recovery for Simple Plex Mouse/Rat TREM-2 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 102 97-107

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat TREM-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse/Rat TREM-2 Cartridge

Mouse/Rat TREM-2 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells 2) is a transmembrane protein that is expressed on newly differentiated and alternatively activated tissue macrophages, immature myeloid dendritic cells, and osteoclasts. TREM2 interacts with and modifies signaling through dendritic cell and osteoclast Plexin A1, a semaphorin receptor. It is active in several settings that involve membrane fusion, including phagocytosis of microbes by macrophages and apoptotic neurons by microglia, and formation of osteoclasts and multinucleated giant cells from macrophages. TREM2 signals through the adaptor protein DAP12 and can associate with the linker protein LAT2 to inhibit macrophage activaation. A soluble isoform of TREM2 antagonizes full-length TREM2 and is elevated in cerebrospinal fluid of patients with active multiple sclerosis.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

Additional TREM2 Products

Product Documents for Simple Plex Mouse/Rat TREM-2 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Mouse/Rat TREM-2 Cartridge

For research use only

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