SMAD1/5/8/9 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56656

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMAD1/5/8/9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against two synthetic peptides corresponding to amino acids 19-33 (GWKQGDEEEKWAEKA) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1.

Specificity

The synthetic peptide immunogens used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAD1/5/8/9 Antibody - BSA Free

Western Blot: SMAD1/5/8/9 Antibody [NB100-56656]

Western Blot: SMAD1/5/8/9 Antibody [NB100-56656]

Western Blot: SMAD1/5/8/9 Antibody [NB100-56656] - Analysis using SMAD1/5/8/9 antibody. Lysate from Saos-2 cells probed with SMAD antibody.
SMAD1/5/8/9 Antibody - BSA Free

Western Blot: SMAD1/5/8/9 Antibody - BSA Free [NB100-56656] -

Radiation effects on TGF‐ beta receptor signaling via noncanonical pathway. (A) Smad1/5/8/9 and pSmad1/5/9 expression in cancer‐associated fibroblasts (CAF) cell lysates analyzed by western blot. (B) quantitative analyses of Smad1/5/8/9 expression in cell samples; (C) quantitative analyses of pSmad1/5/9 expression in cell samples. In B and C, data for pSmad1/5/9 represents mean (+/- SD) values from three different CAF donors. Statistical p‐values between CTR (nonirradiated) and irradiated CAFs were determined using ordinary one‐way ANOVA with Dunnett correction for multiple comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38488488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SMAD1/5/8/9 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAD1/5/8/9

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them.

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Entrez Gene IDs

17125 (Mouse); 25671 (Rat)

Gene Symbol

SMAD1

UniProt

Q15797

Additional SMAD1/5/8/9 Products

Product Documents for SMAD1/5/8/9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMAD1/5/8/9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMAD1/5/8/9 Antibody - BSA Free

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