Sodium Potassium ATPase Alpha 3 Antibody (JE35-24)

Novus Biologicals | Catalog # NBP3-32062

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE35-24 expressed in HEK293
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Product Specifications

Immunogen

Synthetic peptide within Human Sodium Potassium ATPase Alpha 3 aa 1-50 / 1,013. (Uniprot: P13637)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sodium Potassium ATPase Alpha 3 Antibody (JE35-24)

Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) Western Blot: Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) [NBP3-32062]

Western Blot: Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) [NBP3-32062]

Western blot analysis on different lysates with NBP3-32062 at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate (70℃ heat) Lane 3: Mouse brain tissue lysate (no heat) Lane 4: Rat brain tissue lysate Lane 5: Rat brain tissue lysate (no heat) Lane 6: Mouse cerebellum tissue lysate Lane 7: Mouse cerebellum tissue lysate (70℃ heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 112 kDa Observed band size: 100/150 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32062 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for Sodium Potassium ATPase Alpha 3 Antibody (JE35-24)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Alpha 3

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

Long Name

Sodium/potassium-transporting ATPase subunit alpha-3

Alternate Names

ATP1A3

Gene Symbol

ATP1A3

Additional Sodium Potassium ATPase Alpha 3 Products

Product Documents for Sodium Potassium ATPase Alpha 3 Antibody (JE35-24)

Certificate of Analysis

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Product Specific Notices for Sodium Potassium ATPase Alpha 3 Antibody (JE35-24)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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