SPOP Antibody (2N8L8)

Novus Biologicals | Catalog # NBP3-15784

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2N8L8 expressed in HEK293
View all SPOP Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human SPOP (O43791). VFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPOP Antibody (2N8L8)

Western Blot: SPOP Antibody (2N8L8) [NBP3-15784]

Western Blot: SPOP Antibody (2N8L8) [NBP3-15784]

Western Blot: SPOP Antibody (2N8L8) [NBP3-15784] - Western blot analysis of extracts of PC-3 cells, using SPOP Rabbit mAb (NBP3-15784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for SPOP Antibody (2N8L8)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPOP

SPOP encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Alternate Names

HIB homolog 1, Roadkill homolog 1, speckle-type POZ protein, TEF2

Gene Symbol

SPOP

Additional SPOP Products

Product Documents for SPOP Antibody (2N8L8)

Certificate of Analysis

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Product Specific Notices for SPOP Antibody (2N8L8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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