Streptavidin Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB9020N Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB9020N-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Streptavidin Alexa Fluor® 700-conjugated Antibody Summary

Specificity

Detects Streptavidin in Western blot.

Source

Monoclonal Rabbit IgG Clone # 1220C

Purification

Protein A or G purified

Immunogen

Purified streptavidin from Streptomyces avidinii

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Streptavidin

Streptavidin is a 52.8 kDa protein from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetramer forming a beta-barrel with an extraordinarily high affinity for biotin (also known as vitamin B7). It has a dissociation constant (K_d) on the order of ≈10⁻¹⁴ mol/L. The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature and has a high resistance to organic solvents, denaturants, detergents, proteolytic enzymes, and extreme pH ranges or temperatures.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2; Streptavidin