Sulfidefluor 7 AM

Tocris Bioscience | Catalog # 4943

H2S fluorescent probe
Tocris Bioscience

Key Product Details

Description

H2S fluorescent probe

Wavelength

Excitation = 495 nm, Emission = 520 nm
View all Fluorescent Cell Indicators and Sensors »

Product Description

Sulfidefluor 7 AM is a reported to be a hydrogen sulphide (H2S) fluorescent probe. Contains an acetoxymethyl ester, which enhances cell permeability and is hydrolyzed inside the cell to trap the probe. Used to visualize VEGF-induced H2S production in human umbilical vein endothelial cells (HUVECS). Excitation wavelength ~ 495 nm; emission maximum ~ 520 nm.

Licensing Information

Sold under license from the Regents of the University of California

Optical Data

Emission Color Green
λabs 495 nm
λem 520 nm
Cell Permeable Yes
Application Fluorescence Microscopy

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Product Specifications for Sulfidefluor 7 AM

Molecular Weight

685.55

Formula

C31H23N7O12

Storage

Store at -20°C

Chemical Name

N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[(3',6'-diazido-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]glycine (acetyloxy)methyl ester

CAS Number

1416872-50-8

PubChem ID

89306941

InChI Key

QIAZHSBWGCYJGQ-UHFFFAOYSA-N

SMILES

O=C(O1)C2=C(C=CC(C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O)=C2)C31C4=CC=C(N=[N+]=[N-])C=C4OC5=C3C=CC(N=[N+]=[N-])=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.71 20

Preparing Stock Solutions for Sulfidefluor 7 AM

The following data is based on the product molecular weight 685.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.29 mL 36.47 mL 72.93 mL
1 mM 1.46 mL 7.29 mL 14.59 mL
2 mM 0.73 mL 3.65 mL 7.29 mL
10 mM 0.15 mL 0.73 mL 1.46 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 10 publications citing the usage of this product.

Product Documents for Sulfidefluor 7 AM

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Sulfidefluor 7 AM

For research use only

Citations for Sulfidefluor 7 AM

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