TAP Tag Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-31052
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Key Product Details
Species Reactivity
Human, Non-species specific, Yeast
Applications
Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage.
Reactivity Notes
Yeast reactivity reported in scientific literature (PMID: 21115740). Human reactivity reported in scientific literature (PMID: 24525454).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TAP Tag Antibody - Azide and BSA Free
Western Blot: TAP Tag AntibodyAzide and BSA Free [NBP2-31052]
Western Blot: TAP Tag Antibody [NBP2-31052] - Analysis of 10 uL of yeast extract and 7ul of Molecular Weight Protein Ladder per wellWestern Blot: TAP Tag AntibodyAzide and BSA Free [NBP2-31052]
Western Blot: TAP Tag Antibody [NBP2-31052] - High-expressing (clone 1) and low expressing (clone 2) TAP fusion proteins probed with 0.5ug/ml of anti-TAP antibody for 2 hours at RT under standard western blotting conditions. The size of the band varies depending on the size of the fusion protein TAP adds around 20kDa to the size of the protein.Applications for TAP Tag Antibody - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
BBS (pH 8.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: TAP Tag
Alternate Names
tandem affinity purification, tandem affinity purification tag, TAP
Additional TAP Tag Products
Product Documents for TAP Tag Antibody - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for TAP Tag Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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