TBC1D1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30325

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TBC1D1 Primary Antibodies »

Product Specifications

Immunogen

Between 1118 and 1168

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBC1D1 Antibody - BSA Free

TBC1D1 Antibody

Immunoprecipitation:TBC1D1 AntibodyNBP3-30325] -

Immunoprecipitation:TBC1D1 AntibodyNBP3-30325] -Detection of human TBC1D1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D1 antibody NBP3-30325 (lot NBP3-30325-1) used for IP at 6 µg per reaction. TBC1D1 was also immunoprecipitated by rabbit anti-TBC1D1.. For blotting immunoprecipitated TBC1D1, NBP3-30325 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TBC1D1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TBC1D1

TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain and presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2/USP6, yeast Bub2, and CDC16. TBC1D1 and TBC1D4 (AS160) have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. TBC1D1 regulates insulin-mediated GLUT4 translocation through its GAP activity, and is a likely Akt substrate. Mutations in the Tbc1d1 gene lead to some cases of severe human obesity.

Alternate Names

KIAA1108TBC, TBC1, TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1, TBC1 domain family member 1

Gene Symbol

TBC1D1

Additional TBC1D1 Products

Product Documents for TBC1D1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TBC1D1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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