TcBuster-M Transposase Antibody

Catalog #: FAB11511X Datasheet / COA / SDS

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FAB11511X-100UG

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Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TcBuster-M Transposase Antibody Summary

Specificity

Detects TcBuster-M Transposase in Western blots.

Source

Monoclonal Mouse IgG2b Clone # 1077035

Purification

Protein A or G purified

Immunogen

Synthetic Peptide

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TcBuster Transposase

TcBuster is a non-viral gene delivery system that enables stable gene transfer in most cell types. The TcBuster system belongs to the hAT-family of DNA transposons and is derived from the red flour beetle Tribolium castaneum (1). It consists of the TcBuster transposase mRNA and DNA transposon encoding multicistronic cargos for delivery. This antibody binds to the TcBuster-M transposase enzyme, a hyperactive version of TcBuster and carriers out the cut-and-paste reactions for efficient delivery of DNA cargos (2). TcBuster is used in a wide variety of genome engineering applications for both research and commercial purposes. These include, but are not limited to, immune cell therapies (T, NK cells), stem cell therapies for regenerative medicine (iPSCs), and bioproduction of biologics, such as monoclonal antibodies. The TcBuster system is available for purchase from Bio-Techne. For more information, please visit https://www.bio-techne.com/services/gene-engineering-services-tcbuster.

Long Name

Tribolium castaneumTcBuster Transposase Peptide

Alternate Names

GLEAN_06077; TcasGA2_TC006077; TcBuster Transposase; Transposase