Human TGF-alpha Recombinant Protein

Novus Biologicals | Catalog # NBP2-36432

Novus Biologicals
Discontinued Product
NBP2-36432 has been discontinued. View all TGF-alpha products.

Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE
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Product Specifications

Description

A single, non-glycosylated polypeptide corresponding to amino acids Val40 - Ala89 of TGFA.

Source: E. coli

Purity

SDS-PAGE

Endotoxin Level

Less than 1 EU/mg of the rHuTGF-a as determined by LAL method.

Predicted Molecular Mass

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

The ED50, as determined by a cell proliferation assay using murine Balb/3T3 cells, is less than 0.4 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-36432
Preparation Method Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Concentration Lyoph
Reconstitution Reconstitute with 10 mM acetic acid to a final concentration of 0.1 mg/ml.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-alpha

TGF-a is an EGF family cytokine that is expressed as a transmembrane protein and as a shed soluble molecule. It is expressed in pituitary, brain, keratinocytes, macrophages, and many tumors. TGF-a is functional across species and triggers the activation of the EGF receptor. It promotes fibroblast proliferation, keratinocyte migration, epithelial development, and angiogenesis.

Long Name

Transforming Growth Factor alpha

Alternate Names

TGFA, TGFalpha

Gene Symbol

TGFA

Additional TGF-alpha Products

Product Documents for Human TGF-alpha Recombinant Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human TGF-alpha Recombinant Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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