TMPRSS11E Overexpression Lysate

Name: TMPRSS11E Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-07384
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-07384

Submit a review

Product Documents

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for TMPRSS11E Overexpression Lysate

TMPRSS11E Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC220553

Accession#: NM_014058

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Theoretical MW

47.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for TMPRSS11E Overexpression Lysate

Western Blot: TMPRSS11E Overexpression Lysate [NBP2-07384]

Western Blot: TMPRSS11E Overexpression Lysate (Adult Normal) [NBP2-07384] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMPRSS11E.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: TMPRSS11E

TMPRSS11E is a member of a larger family of membrane attached serine proteases, a poorly defined group that includes TMPRSS11A, B, C, D, E, F, Hepsin, Corin, Matriptase 1, 2 and 3. The highest degree of identity is with TMPRSS11A, which shares 43% identity at the amino acid level. TMPRSS11E has a domain structure of an aminoterminal cytoplasmic domain, followed by a transmembrane domain, a SEA domain (Sea urchin sperm protein, Enterokinase, Agrin), a short spacer, then the trypsin like serine protease domain. The SEA domain is thought to play a role in carbohydrate binding in the analogous protein sequences where it is found, but the role in TMPRSS11E is unclear. The cleavage of the Arg191 Ile192 bond liberates the catalytic domain. TMPRSS11E has been shown to cleave fibronectin, gelatin, casein and pro uPA in vitro, and to form complexes with serpinA5 and serpinE1.

Alternate Names

DESC1, FLJ75331, FLJ94490, MGC141972, MGC141974, serine 11E2, serine protease DESC1, TMPRSS11E2, transmembrane protease serine 11E, transmembrane protease serine 11E2, transmembrane protease, serine 11E, UNQ742/PRO1461

Gene Symbol

TMPRSS11E

Additional TMPRSS11E Products

Product Documents for TMPRSS11E Overexpression Lysate

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TMPRSS11E Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customer Reviews for TMPRSS11E Overexpression Lysate

There are currently no reviews for this product. Be the first to review TMPRSS11E Overexpression Lysate and earn rewards!

Have you used TMPRSS11E Overexpression Lysate?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image