Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 250 and 300 of human Sjogren syndrome antigen A1 using the numbering given in entry NP_003132.2 (GeneID 6737).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TRIM21/SSA1 Antibody - BSA Free (NBP1-37009) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRIM21/SSA1 Antibody - BSA Free

Immunoprecipitation: TRIM21/SSA1 Antibody [NBP1-37009]

Immunoprecipitation: TRIM21/SSA1 Antibody [NBP1-37009]

Immunoprecipitation: TRIM21/SSA1 Antibody [NBP1-37009] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SSA1 antibody used for IP at 10 mcg/mg lysate. SSA1 was also immunoprecipitated by rabbit anti-SSA1 antibody NBP1-40338 which recognizes a downstream epitope.

Applications for TRIM21/SSA1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

5-15 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRIM21

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Long Name

Tripartite Motif-containing Protein 21

Alternate Names

RNF81, Ro52, SSA1

Entrez Gene IDs

6737 (Human)

Gene Symbol

TRIM21

Additional TRIM21 Products

Product Documents for TRIM21/SSA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TRIM21/SSA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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