Triptolide

Tocris Bioscience | Catalog # 3253

Inhibits RNAPII-mediated transcription; antitumor, anti-inflammatory and immunosuppressive
Tocris Bioscience

Key Product Details

Description

Inhibits RNAPII-mediated transcription; antitumor, anti-inflammatory and immunosuppressive
View all NFkB/IkB Inhibitors »

Product Description

Triptolide inhibits DNA-dependent ATPase activity of XBP and induces inhibition of RNA polymerase II (RNAPII)-mediated transcription (IC50 = 200 nM). Selective for RNAPII over RNAPI and RNAPIII. Triptolide blocks RNA synthesis in HeLa cells (IC50 = 62 nM); exhibits potent antiproliferative activity in 60 cancer cell lines (average IC50 = 12 nM) and induces apoptosis by blocking TNF-α-mediated c-IAP1 and c-IAP2 induction. Also displays immunosuppressive and anti-inflammatory activity.

Product Specifications for Triptolide

Molecular Weight

360.40

Formula

C20H24O6

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)-3b,4,4a,6,6a,7a,7b,8b,9,10-Decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)trisoxireno[4b,5:6,7:8a,9]phenanthro[1,2-c]furan-1(3H)-one

CAS Number

38748-32-2

PubChem ID

107985

InChI Key

DFBIRQPKNDILPW-CIVMWXNOSA-N

SMILES

O=C1C2=C([C@@](C[C@H]5[C@]4(O5)[C@](O6)3[C@@H]6[C@H]7[C@](C(C)C)(O7)[C@H]4O)([H])[C@]3(C)CC2)CO1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.21 20

Preparing Stock Solutions for Triptolide

The following data is based on the product molecular weight 360.40.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 13.87 mL 69.37 mL 138.73 mL
1 mM 2.77 mL 13.87 mL 27.75 mL
2 mM 1.39 mL 6.94 mL 13.87 mL
10 mM 0.28 mL 1.39 mL 2.77 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 48 publications citing the usage of this product.

Product Documents for Triptolide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Triptolide

For research use only

Customer Reviews for Triptolide (1)

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  • Works well
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: U2OS
    Verified Customer | Posted 06/22/2020
    I used short treatments with the transcription inhibitors triptolide. These inhibited ongoing RNA synthesis and increased replication fork speeds specifically in the presence of JQ1
    triptolide (1 μM)
    Triptolide 3253

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