Tunicamycin

Tocris Bioscience | Catalog # 3516

Antibiotic; GlcNAc phosphotransferase inhibitor
Tocris Bioscience

Key Product Details

Description

Antibiotic; GlcNAc phosphotransferase inhibitor
View all Other Transferase Inhibitors »

Product Description

Tunicamycin is an antibiotic; inhibits GlcNAc phosphotransferase (GPT). Blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis. Activity induces ER stress and causes G1 arrest; can be used to induce autophagy. Tunicamycin contains four main components as follows:
  • Homolog A, n=8, C37H60N4O16, molecular weight = 816.90
  • Homolog B, n=9, C38H62N4O16, molecular weight = 830.93
  • Homolog C, n=10, C39H64N4O16, molecular weight = 844.95
  • Homolog D, n=11, C40H66N4O16, molecular weight = 858.99
The composition of this product will vary from batch to batch and can be found on the relevant certificate of analysis.

Product Specifications for Tunicamycin

Molecular Weight

844.95

Formula

C39H64N4O16 (tunicamycin C, n=10)

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

Tunicamycin from Streptomyces sp.

CAS Number

11089-65-9

PubChem ID

90488851

InChI Key

ZOCXUHJGZXXIGQ-SQXRCPDGSA-N

SMILES

O=C(C=C3)NC(N3[C@@H]2O[C@@H]([C@@H](O)[C@H]2O)[C@H](O)C[C@@H]1[C@H](O)[C@H](O)[C@@H](NC(/C=C/CCCCCCCCCCC(C)C)=O)[C@H](OC4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4NC(C)=O)O1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.25 50

Preparing Stock Solutions for Tunicamycin

The following data is based on the product molecular weight 844.95.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.37 mL 11.84 mL 23.67 mL
2.5 mM 0.47 mL 2.37 mL 4.73 mL
5 mM 0.24 mL 1.18 mL 2.37 mL
25 mM 0.05 mL 0.24 mL 0.47 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 103 publications citing the usage of this product.

Product Documents for Tunicamycin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Tunicamycin

For research use only

Customer Reviews for Tunicamycin (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 的 4 reviews Showing All
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  • Good product
    Name: P Kumar
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: AC16
    Verified Customer | Posted 04/19/2021
    examine effect in AC16 cell line.
    Tunicamycin 3516
  • Usage Information
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: KMS11 or OPM2
    Verified Customer | Posted 06/20/2020
    I dissolved tunicamycin in DMSO. I treated cells for 16 hr.
    5 μg/ml
    Tunicamycin 3516
  • Worked at once
    Name: Bibha Dahal
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human primary astrocytes
    Verified Customer | Posted 01/08/2020
    It took sometime to dissolve so you might want to warm once you add DMSO to tunicamycin in water bath.
    I dissolved tunicamycin in DMSO. I added tunicamycin at various concentrations (2uM, 4uM, 6uM, and 10uM) for 24 hours in human primary astrocytes and incubated at 37 C and 5% CO2. Next day, I collected lystates and ran a western blot. The first four lanes are tunicamycin added samples in increasing concentration and the last two lanes are without added. I looked for PERK activation using p-PERK antibody.
    Tunicamycin 3516
  • Tunicamycin Usage Information
    Name: Chao Li
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human Intestine Subepithelial myofibroblasts
    Verified Customer | Posted 01/08/2018
    It is working well and easily dissolved into DMSO. I used 2.5, 5 µg/ml of Tunicamycin with serum free culture medium to treat the cells for 8h, 16h, 24h, and 48h.

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