UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free

Novus Biologicals | Catalog # NBP3-43536

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SAIC-41C-6

Format

BSA Free
View all UbcH10/UBE2C Primary Antibodies »

Product Specifications

Immunogen

Peptide "LSLEFPSGYPYNAPTVK" derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH. (Uniprot# O00762)

Specificity

Recognises human UbcH10/UBE2C.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free

UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free

Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free [NBP3-43536] -

Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free [NBP3-43536] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-43536 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free

Immunocytochemistry /Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free [NBP3-43536] -

Immunocytochemistry /Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free [NBP3-43536] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with NBP3-43536 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43536, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

Applications for UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH10/UBE2C

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

UBE2C

Gene Symbol

UBE2C

Additional UbcH10/UBE2C Products

Product Documents for UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UbcH10/UBE2C Antibody (SAIC-41C-6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway
Ubiquitination Cascade Pathway Thumbnail