Recombinant Human UMPS His Protein

Novus Biologicals | Catalog # NBP2-22934

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all UMPS Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-480 of Human UMPS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UMPS His Protein

SDS-PAGE: Recombinant Human UMPS His Protein [NBP2-22934]

SDS-PAGE: Recombinant Human UMPS His Protein [NBP2-22934]

SDS-Page: UMPS Protein [NBP2-22934] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP2-22934
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UMPS

Uridine 5'-monophosphate synthase, also known as UMPS, is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. Unlike prokaryotes, UMPS in eukaryotes combines the orotate phosphoribosyltransferase and the orotidine-5'-monophosphate (OMP) decarboxylase activities into a single protein. The union of these two enzymes is thought to stabilize the catalytic centers due to the low molar concentration of the protein in mammalian cells. Defects in this gene are the cause of hereditary orotic aciduria. Recombinant human UMPS protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

EC 4.1.1.23, OMPdecase, OPRT, OPRTase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, UMP synthase, uridine 5'-monophosphate synthase, uridine monophosphate synthetase

Gene Symbol

UMPS

Additional UMPS Products

Product Documents for Recombinant Human UMPS His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human UMPS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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