UNveil Unstained Protein Ladder

Novus Biologicals | Catalog # NBP3-33175

Novus Biologicals
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Key Product Details

Applications

Western Blot

Product Summary for UNveil Unstained Protein Ladder

The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.

(12 unstained bands, 10-200 kDa)
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Product Specifications

Application Notes

3 ul or 5 ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 ul per well for general Western transferring

Scientific Data Images for UNveil Unstained Protein Ladder

UNveil Unstained Protein Ladder

Western Blot: UNveil Unstained Protein Ladder [NBP3-33175] -

Western Blot: UNveil Unstained Protein Ladder [NBP3-33175] - The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.

Formulation, Preparation, and Storage

Formulation

20 mM Tris-phosphate (pH 7.5), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Product Documents for UNveil Unstained Protein Ladder

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UNveil Unstained Protein Ladder

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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