UNveil Unstained Protein Ladder
Novus Biologicals | Catalog # NBP3-33175
Loading...
Key Product Details
Applications
Western Blot
Product Summary for UNveil Unstained Protein Ladder
The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
(12 unstained bands, 10-200 kDa)
(12 unstained bands, 10-200 kDa)
Loading...
Product Specifications
Application Notes
3 ul or 5 ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 ul per well for general Western transferring
Scientific Data Images for UNveil Unstained Protein Ladder
Western Blot: UNveil Unstained Protein Ladder [NBP3-33175] -
Western Blot: UNveil Unstained Protein Ladder [NBP3-33175] - The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.Formulation, Preparation, and Storage
Formulation
20 mM Tris-phosphate (pH 7.5), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C. Avoid freeze-thaw cycles.
Product Documents for UNveil Unstained Protein Ladder
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UNveil Unstained Protein Ladder
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Customer Reviews for UNveil Unstained Protein Ladder
There are currently no reviews for this product. Be the first to review UNveil Unstained Protein Ladder and earn rewards!
Have you used UNveil Unstained Protein Ladder?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs
No product specific FAQs exist for this product.
View all FAQs for Supplemental Kits and ReagentsLoading...