Recombinant Human UROS His Protein

Novus Biologicals | Catalog # NBP1-72312

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-265 of Human UROS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKVLLLKDAK EDDCGQDPYI RELGLYGLEA TLIPVLSFEF LSLPSFSEKL SHPEDYGGLI FTSPRAVEAA ELCLEQNNKT EVWERSLKEK WNAKSVYVVG NATASLVSKI GLDTEGETCG NAEKLAEYIC SRESSALPLL FPCGNLKREI LPKALKDKGI AMESITVYQT VAHPGIQGNL NSYYSQQGVP ASITFFSPSG LTYSLKHIQE LSGDNIDQIK FAAIGPTTAR ALAAQGLPVS CTAESPTPQA LATGIRKALQ PHGCC

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UROS His Protein

SDS-PAGE: Recombinant Human UROS His Protein [NBP1-72312]

SDS-PAGE: Recombinant Human UROS His Protein [NBP1-72312]

SDS-Page: Recombinant Human UROS His Protein [NBP1-72312] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP1-72312
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UROS

UROS, also known as Uroporphyrinogen III synthase, is an enzyme involved in the fourth step of porphyrin metabolism, involved in the conversion of hydroxymethyl bilane into uroporphyrinogen III. Defects in this protein can cause molecular lesions that lead to the autosomal recessive Gunther disease, also known as congenital erythropoietic porphyria (CEP). Recombinant human UROS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EC 4.2.1.75, hydroxymethylbilane hydrolyase, Hydroxymethylbilane hydrolyase [cyclizing], UROIIIS, uroporphyrinogen III synthase, Uroporphyrinogen-III cosynthase, uroporphyrinogen-III synthase

Gene Symbol

UROS

Additional UROS Products

Product Documents for Recombinant Human UROS His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human UROS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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