Virginia Orange, Haloalkane

Tocris Bioscience | Catalog # 8959

Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate
Tocris Bioscience
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Key Product Details

Description

Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate

Wavelength

Excitation = 555 nm, Emission = 581 nm
View all Self-labeling Tags »

Product Description

Key Information: Virginia Orange, Haloalkane is pH sensitive a red cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.

Used for: endosomal uptake assays, protein delivery experiments and evaluation of protein cargo internalization.

Application: Live-cell imaging as a self-labeling tag substrate; fluorescent microscopy and flow cytometry.

Properties and Photophysical Data: shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 555 nm and 581 nm, respectively; quantum yield = 0.40; extinction coefficient = 90,900 M-1cm-1; pH sensitive fluorescence h = 1.46. PKa = 6.25. Colourless and non-fluorescent at low pH.

Optical Data

Emission Color Red
λabs 555 nm
λem 581 nm
Extinction Coefficient (ε) 90,900 M-1cm-1
Quantum Yield (φ) 0.4
Cell Permeable Yes
pH Sensitivity 1.46
Application Fluorescence microscopy, flow cytometry

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Product Specifications for Virginia Orange, Haloalkane

Molecular Weight

644.11

Formula

C34H36ClF2NO7

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(2,7-difluoro-6-hydroxy-10,10-dimethyl-3-oxo-3,10-dihydroanthracen-9-yl)benzoic acid

InChI Key

LSFLCNCAFZHNTQ-UHFFFAOYSA-N

SMILES

O=C(C1=CC=C(C(C(C2=CC(F)=C(C=C2C3(C)C)O)=C(C3=C4)C=C(F)C4=O)=C1)C(O)=O)NCCOCCOCCCCCCCl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.44 10

Preparing Stock Solutions for Virginia Orange, Haloalkane

The following data is based on the product molecular weight 644.11.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 15.53 mL 77.63 mL 155.25 mL
0.5 mM 3.11 mL 15.53 mL 31.05 mL
1 mM 1.55 mL 7.76 mL 15.53 mL
5 mM 0.31 mL 1.55 mL 3.11 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Virginia Orange, Haloalkane

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Virginia Orange, Haloalkane

For research use only

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