Infection by mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication, and are therefore potential targets for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor.
wnvNS3 Protease Antibody [mFluor Violet 610 SE]
Novus Biologicals | Catalog # AF2907MFV610
Key Product Details
Species Reactivity
Viral
Applications
Western Blot, Immunoprecipitation
Label
mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L
Gly1506-Leu1689
Accession # ABD85079
Gly1506-Leu1689
Accession # ABD85079
Specificity
Detects West Nile Virus NS3 Protease in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for wnvNS3 Protease Antibody [mFluor Violet 610 SE]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: wnvNS3 Protease
Long Name
West Nile Virus NS3 Protease
Gene Symbol
POLY
Additional wnvNS3 Protease Products
Product Documents for wnvNS3 Protease Antibody [mFluor Violet 610 SE]
Product Specific Notices for wnvNS3 Protease Antibody [mFluor Violet 610 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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