X-Gal

Tocris Bioscience | Catalog # 5510

Used in cloning procedures with IPTG
Tocris Bioscience
Discontinued Product
5510 has been discontinued. View all Buffers, Chelators and Reagents products.

Key Product Details

Description

Used in cloning procedures with IPTG
View all Buffers, Chelators and Reagents »

Product Description

Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.

Product Specifications for X-Gal

Molecular Weight

408.63

Formula

C14H15BrClNO6

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside

CAS Number

7240-90-6

PubChem ID

65181

InChI Key

OPIFSICVWOWJMJ-AEOCFKNESA-N

SMILES

ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40.86 100

Preparing Stock Solutions for X-Gal

The following data is based on the product molecular weight 408.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.45 mL 12.24 mL 24.47 mL
5 mM 0.49 mL 2.45 mL 4.89 mL
10 mM 0.24 mL 1.22 mL 2.45 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

There are currently no references for this product.

Product Documents for X-Gal

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for X-Gal

For research use only

Customer Reviews for X-Gal (1)

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  • X gal for Blue White Screening
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 02/04/2019
    Bacterial cells were plated with L-agar, Ampillicin and X-gal solution at two different concentrations (5 and 50 µl) and a negative control containing only sterile water was set up. The plates were allowed to grow for 48 hours and counted for white and blue colonies.
    X-Gal 5510

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