Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor)

Novus Biologicals | Catalog # NBP2-29397

Novus Biologicals
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Key Product Details

Applications

Functional Assay

Product Specifications

Details of Functionality

Molecular Weight: 654
Formula: C30H43N4O11F
Mass Spec: M+1=655.1
TLC: EtOAc: 100%, Rf:0.3
NMR: All functional groups are present

Application Notes

1. This inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate the free carboxyl groups. Please contact us for more details.
2. For in-vivo or in-vitro experiments extending longer than 12 hrs, fresh inhibitor may have to be added to culture medium or injected into animals.

Formulation, Preparation, and Storage

Formulation

Form:Yellow solid
Make a stock solution of 5, 10 or 20 mM in high purity DMSO (>99.9%).

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Z-IE(OMe)TD(OMe)-FMK

Members of the caspase family play key roles in apoptosis and inflammation. Z-IE(OMe)TD(OMe)-FMK is a cell-permeable caspase peptide inhibitor that irreversibly binds to the catalytic site of caspases proteases, and inhibits caspase mediated apoptosis by preventing the processing of pro-caspases to their active forms (reviewed in 1-3). Z-IETD-FMK was first described is an irreversible and cell permeable inhibitor of Caspase 8. The Z-IE(OMe)TD(OMe)-FMK peptide is O-methylated in the P1 and P3 positions providing enhanced stability and increased cell permeability. Z-IE(OMe)TD(OMe)-FMK (Z-IETD-FMK) is typically used in assays to inhibit apoptosis. Z-IETD-FMK has been used in many different types of apoptosis assays and published using a number of model system. Users may want to consult the literature for additional information regarding applications for Z-IETD D-FMK. Z-IETD-FMK is recommended as a search term for identifying references in PubMed using this peptide inhibito

Alternate Names

C30H43N4O11F, Z-IE(OMe)TD(OMe)-FMK, Z-IETD-FMK, Z-IETD-FMK [Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK

Additional Z-IE(OMe)TD(OMe)-FMK Products

Product Documents for Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Citations for Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor)

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Protocols

View specific protocols for Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor) (NBP2-29397):

Product Handling - Assay Method (NBP2-29397):
Materials:
Dissolve 5 mg of Z-IETD-FMK in DMSO to get appropriate concentration:
765 ml DMSO = 10 mM
383 ml DMSO = 20 mM
1530 ml DMSO = 5 mM

Method:
Add 2 ml of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of 0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease inhibitors. 2 ml of 10 mM stock in 1 ml medium = 20 mM final Z-IETD-FMK concentration.