Z-Val-Ala-Asp (OMe)-FMK Inhibitor
Novus Biologicals | Catalog # NBP2-29392
Product Summary for Z-Val-Ala-Asp (OMe)-FMK Inhibitor
Product Specifications
Details of Functionality
Chromatography: TLC:EtOAc:80, Hex: 20, Single Spot, Rf:0.3, Single spot>95%
H NMR: All functional groups are present
HPLC: >90%
Peptide content: 81%
Formula: C22H30N3O7F
Molecular Weight: 467
Formulation, Preparation, and Storage
Formulation
Make a stock solution of 20 mM in high purity DMSO (>99.9%).
Concentration
Shipping
Storage
Background: Z-Val-Ala-Asp (OMe)-FMK
Alternate Names
Additional Z-Val-Ala-Asp (OMe)-FMK Products
Product Documents for Z-Val-Ala-Asp (OMe)-FMK Inhibitor
Certificate of Analysis
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Product Specific Notices for Z-Val-Ala-Asp (OMe)-FMK Inhibitor
This inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate the free carboxyl groups.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.
Citations for Z-Val-Ala-Asp (OMe)-FMK Inhibitor
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Protocols
View specific protocols for Z-Val-Ala-Asp (OMe)-FMK Inhibitor (NBP2-29392):
Materials:
Dissolve 1mg of Z-Val-Ala-Asp(OMe)-FMK in DMSO to get appropriate concentration:
107 ul DMSO = 20mM
214 ul DMSO = 10 mM
428 ul DMSO = 5 mM
Method:
Add 2 ul of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of
0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease
inhibitors.
FAQs for Z-Val-Ala-Asp (OMe)-FMK Inhibitor
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Q: For assays with recombinant enzymes, esterase should be added: which type of esterase, how much, and can you recommend a specific enzyme?
A: When this peptide FMK inhibitor or other OMe peptide inhibitors are used with purified caspases, the ester groups are not rapidly hydrolyzed and the inhibitor-enzyme reaction rate may be reduced.. The ester groups can be removed by pretreating with esterase. For example, dilute the FMK inhibitor stock (or DMSO only for a control) 10-fold into 10 mM borate buffer, pH 8.0, containing 1 unit of esterase (e.g., carboxylic acid ester hydrolase which is available from different vendors) and incubate for 15 minutes on ice. Then dilute an additional 100-fold into the final reaction mixture and incubate again for 15 minutes prior to adding the purified caspase substrate. This is a general protocol, and you may also be able to find other suggestions in the scientific literature.