Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
Discontinued Product
3448-020-CH has been discontinued.
View all Basement Membrane Extracts products.
Key Product Details
Features
Optimized and standardized solutions for the isolation of intact 3-D structures from Basement Membrane Extract (BME) or Laminin I hydrogels. This product is also a part of the Cultrex 3-D Culture Cell Harvesting Kit.
Product Summary for Cultrex 3-D Culture Cell Harvest Buffers
Why Use Cultrex 3-D Culture Cell Harvest Buffers?
3-D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels. Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex 3-D Culture Cell Harvest Buffers depolymerize BME and Laminin I hydrogels, non-enzymatically, providing an optimized and standardized solution for the isolation of 3-D structures.
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Kit Contents for Cultrex 3-D Culture Cell Harvest Buffers
- 3-D Culture 10X Cell Harvesting Buffer
- 3-D Culture 10X Cell Wash Buffer
Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Storage
Store the unopened product at room temperature.
Background: Basement Membrane Extracts
Additional Basement Membrane Extracts Products
Product Documents for Cultrex 3-D Culture Cell Harvest Buffers
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
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Application: Immunoassay StandardVerified Customer | Posted 04/01/2018
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