Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
Cultrex 3-D Culture Cell Harvesting Kit
R&D Systems | Catalog # 3448-020-K
Discontinued Product
3448-020-K has been discontinued.
View all Basement Membrane Extracts products.
Key Product Details
Features
Optimized and standardized kit for the isolation of intact 3-D structures from Basement Membrane Extract (BME) Matrix or Laminin I hydrogels and preparation of cell lysates for subsequent biochemical analysis.
Product Summary for Cultrex 3-D Culture Cell Harvesting Kit
Why Use Cultrex 3-D Culture Cell Harvesting Kit?
3-D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels. Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex 3-D Culture Cell Harvesting Kit depolymerizes BME and Laminin I hydrogels, non-enzymatically, providing an optimized and standardized solution for the isolation of 3-D structures and preparation of cell lysates for subsequent biochemical analysis.
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Kit Contents for Cultrex 3-D Culture Cell Harvesting Kit
- 3-D Culture 10X Cell Harvesting Buffer
- 3-D Culture 10X Cell Wash Buffer
- 3-D Culture Sample Buffer
- 3-D Culture 10X Loading Buffer
- Human Anti-G3PDH Antibody
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
The components for this kit may require different storage temperatures. Upon receipt, store products immediately at the temperature recommended on the product labels.
Background: Basement Membrane Extracts
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Product Documents for Cultrex 3-D Culture Cell Harvesting Kit
Certificate of Analysis
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Citations for Cultrex 3-D Culture Cell Harvesting Kit
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Customer Reviews for Cultrex 3-D Culture Cell Harvesting Kit (4)
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4 Customer Ratings
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Application: In vivo studyVerified Customer | Posted 01/13/2020
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Application: In vivo studyVerified Customer | Posted 05/27/2019
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Application: Digest OrganoidsVerified Customer | Posted 03/13/2019Used to digest Organoids to make Cell lysates for Western Blot to look at protein expression.
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Application: Cell ProliferationVerified Customer | Posted 04/01/2018
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